LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1889..1985) /label=attL2 /note="attL2 (c)" misc_signal complement(2000..2019) /note="T7 Promoter/priming site (c)" primer_bind 2027..2043 /note="M13 Reverse priming site" gene 2156..2965 /note="Kanamycin resistance gene" rep_origin 3086..3759 /note="pUC origin" vector join(1897..3762,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1882 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="IMAGE:100061932" /clone_lib="NIH_MGC_425" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 without stop codon" source 692..1882 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..1882 /gene="HS3ST4" /gene_synonym=30ST4 /gene_synonym=3OST4 /db_xref="GeneID:9951" /db_xref="HGNC:5200" /db_xref="MIM:604059" CDS 692..1882 /dnas_title="heparan sulfate (glucosamine) 3-O-sulfotransferase 4" /gene="HS3ST4" /gene_synonym=30ST4 /gene_synonym=3OST4 /codon_start=1 /product="heparan sulfate (glucosamine) 3-O-sulfotransferase 4" /protein_id="AAI56388.1" /db_xref="GI:162318996" /db_xref="GeneID:9951" /db_xref="HGNC:5200" /db_xref="MIM:604059" /translation="MARWPAPPPPPPPPPPLAAPPPPGASAKGPPARKLLFMCTLSLS VTYLCYSLLGGSGSLQFPLALQESPGAAAEPPPSPPPPSLLPTPVRLGAPSQPPAPPP LDNASHGEPPEPPEQPAAPGTDGWGLPSGGGGAQDAWLRTPLAPSEMITAQSALPERE AQESSTTDEDLAGRRAANGSSERGGAVSTPDYGEKKLPQALIIGVKKGGTRALLEAIR VHPDVRAVGVEPHFFDRNYEKGLEWYRNVMPKTLDGQITMEKTPSYFVTNEAPKRIHS MAKDIKLIVVVRNPVTRAISDYTQTLSKKPEIPTFEVLAFKNRTLGLIDASWSAIRIG IYALHLENWLQYFPLSQILFVSGERLIVDPAGEMAKVQDFLGLKRVVTEKHFYFNKTK GFPCLKKPEDSSAPRCLGKSKGRTHPRIDPDVIHRLRKFYKPFNLMFYQMTGQDFQWE QEEGDK" misc_feature 692..1882 /note="HS3ST4 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccaattccct ctggcgctgc aggagtcgcc 721 gggcgccgcc gccgagcccc cgccgagccc gccgccaccc tctctgctgc ctacccccgt 781 gcgcctcggc gccccctcgc agccgcccgc gccgccgccg ctggacaacg cgagccacgg 841 ggagccgccc gagcccccag agcagccagc cgcccccggg accgacggct gggggctgcc 901 gagcggcggc ggaggcgccc aggacgcctg gctccggacc ccgctggccc ccagcgagat 961 gatcacggct cagagcgcgc tgccggagag ggaagcgcag gagtccagca ccaccgacga 1021 ggatctcgca ggccggagag cggccaacgg gagcagcgag aggggcggcg ccgtcagcac 1081 ccccgactat ggggagaaga agctgccaca ggcgctcatc atcggggtca agaaaggagg 1141 gacccgcgcg ctgctggagg cgatccgcgt gcacccggac gtgcgggcgg tgggcgtaga 1201 gccgcacttc ttcgacagga actacgaaaa ggggttggag tggtacagaa atgtgatgcc 1261 caagactttg gatgggcaaa taaccatgga gaagactcca agttactttg tgacaaatga 1321 ggctcccaag cgcattcact ccatggccaa ggacatcaaa ctgattgtgg tggtgagaaa 1381 ccccgtgacc agggccatct ctgactacac gcagacactg tcaaagaaac ccgagatccc 1441 cacctttgag gtgctggcct tcaaaaaccg gaccctcggg ctgatcgatg cttcctggag 1501 tgccattcga atagggatct atgcgctgca tctggaaaac tggctccagt atttccccct 1561 ctcccagatc ctctttgtca gtggtgagcg actcattgtg gaccccgccg gggaaatggc 1621 caaagtacag gattttctag gcctcaaacg tgttgtgact gagaagcatt tctatttcaa 1681 caaaaccaag gggttccctt gcctaaagaa gccagaagac agcagtgccc cgaggtgctt 1741 aggcaagagc aaaggtcgga ctcatcctcg cattgaccca gatgtcatcc acagactgag 1801 gaaattctac aaacccttca acttgatgtt ttaccaaatg actggtcaag attttcagtg 1861 ggaacaggaa gagggtgata aaTAGGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 1921 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 1981 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2041 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2101 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2161 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2221 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2281 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2341 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2401 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 2461 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 2521 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 2581 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 2641 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 2701 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 2761 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 2821 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 2881 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 2941 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3001 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3061 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3121 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3181 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3241 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3301 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3361 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3421 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3481 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3541 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3601 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3661 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 3721 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //